Lipid Phase Transitions and Procoagulant Activity

Phase Transitions in Lipid Membranes

The seminar treats phase transitions in lipid membranes, with the focus on the main lipid bilayer phase transition. It reveals the microscopic picture and reviews the key factors leading to the presented phenomena. A general discussion on models is made and some concrete examples are presented in more detail. Finally, experimental approaches to study the phase behavior of lipid membranes are di...

Phase-separation transitions in asymmetric lipid bilayers

Morphological transitions of phase separation associated with the asymmetry of lipid composition were investigated using micrometer-sized vesicles of lipid bilayers made from a lipid mixture. The complete macro-phase-separated morphology undergoes a transition to a micro-phase-separation-like morphology via a lorate morphology as a metastable state. The transition leads to the emergence of mono...

Periodic structures in lipid monolayer phase transitions.

Periodic patterns are observed when supported lipid monolayers doped with low concentrations of fluorescent lipid probes are observed with epi-fluorescence microscopy. Monolayers of dipalmitoyl phosphatidylcholine were examined on air-water interfaces and also on alkylated glass coverslips. The patterns are formed by periodic arrays of solid-phase lipid domains in equilibrium with fluid-phase l...

Procoagulant activity in gastroenterology.

Calorimetric evidence for phase transitions in spin-label lipid bilayers.

Dispersions of pure, spin-label phosphatidylcholines in aqueous buffer have been investigated with the Privalov high-sensitivity differential scanning calorimeter. The lipids studied are mixed-chain ones in which C-2 of glycerol bears a spin-label derivative of stearic acid and the fatty acid group at C-1 is palmitate. A well-defined phase transition is observed at 30.3-30.7 degrees C for the p...